Adewusi et al., AfrJTraditComplementAlternMed.(2013)10(4):6‐11 http://dx.doi.org/10.4314/ajtcam.v10i4.2
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EFFECT OF FOUR MEDICINAL PLANTS ON AMYLOID-β INDUCED NEUROTOXICITY IN SHSY5Y CELLS Emanuel A. Adewusia, Gerda Foucheb and Vanessa Steenkampa a
Department of Pharmacology, Faculty of Health Sciences, University of Pretoria, Private Bag X323, Arcadia 0007, South Africa, bNatural Product Chemistry Group, Council for Scientific and Industrial Research, PO Box 395, Pretoria 0001 *E-mail: [emailprotected], [emailprotected], [emailprotected]
Abstract Amyloid-beta peptide (Aβ) is implicated in the pathogenesis of Alzheimer’s disease (AD), a neurodegenerative disorder. This study was designed to determine the effect of four medicinal plants used to treat neurodegenerative diseases on Aβinduced cell death. Cytotoxicity of the ethanol extracts of the plants was determined against SH-SY5Y (human neuroblastoma) cells which were untreated, as well as toxically induced with Aβ, using the MTT and neutral red uptake assays. Cell viability was reduced to 16% when exposed to 20 μM Aβ25-35 for 72 h. The methanol extract of the roots of Ziziphus mucronata Willd., Lannea schweinfurthii (Engl.) Engl. and Terminalia sericea Burch. ex DC., were the least toxic to the SH-SY5Ycells at the highest concentration tested (100 μg/ml). All four plants tested were observed to reduce the effects of Aβ-induced neuronal cell death, indicating that they may contain compounds which may be relevant in the prevention of AD progression. Key words: Amyloid-beta peptide, medicinal plants, neurodegenerative disorders, neurotoxicity, SH-SY5Y cells
Introduction Neurodegenerative disease is a term applied to a variety of conditions which result from a chronic breakdown and deterioration of neurons, particularly those of the central nervous system (CNS). These neurons may accumulate aggregated proteins which cause dysfunction (Houghton and Howes, 2005). Alzheimer’s disease (AD) is a neurodegenerative disorder and the most common cause of age-related intellectual impairment occurring after the age of sixty (Silva et al., 2004). Patients with AD suffer from marked reduction of cholinergic neuronal function in those areas of the brain responsible for higher mental functions, which partially account for the impairments in activities of daily life (Coyle and Kershaw, 2001). One of the major pathological features of AD is the abundant presence of amyloid plaques in the brain tissues of affected individuals (Pereira et al., 2005). These plaques are made up of amyloid-β proteins (Aβ), which are neurotoxic and are actively involved in the neuronal degeneration that occurs in AD (Ji et al., 2006). As a result, modulation of its toxicity has been identified to be an important therapeutic approach to control the onset of AD (Findeis, 2000; Kawahara and Kuroda, 2000; Ji et al., 2006). In the in vitro model of AD, Aβ has been used to initiate neurotoxicity in various types of cultured cells (Puttfarcken et al., 1996; Boyd-Kimball et al., 2004; Martin et al., 2004; Limpeanchob et al., 2008). In spite of this mechanistic understanding of the pathophysiology of AD, current medication for AD is very limited and the available ones have several side effects including gastrointestinal disturbances and problems associated with bioavailability (Melzer, 1998, Schulz, 2003). Natural products have provided an alternative strategy for AD therapy as they are usually safer and have fewer adverse effects than chemically synthesised drugs (Kang et al, 2011). Recent findings have shown that natural products have the potential not only to prevent Aβ toxicity, but also to prevent the production of Aβ (Yu et al., 2005). For example, resveratrol (derived from red grape), curcumin (derived from spice turmeric) and (˗)-epigallocatechin-3-gallate (derived from green tea) have been reported to reduce the effect of Aβ in the cerebral cortex; curcumin is reported to have the ability to bind small Aβ peptides to block Aβ aggregation as well as fibril and oligomer Aβ formation (Yu et al., 2005; Kang et al., 2011). In southern Africa, approximately 3500 species of higher plants are used as traditional medicines (Gericke, 2002). These plants contain chemical substances with interesting pharmacological effects and several of these plants are used to treat neurological and age-related disorders (Gericke, 2002). In a previous study, a number of plants, including Ziziphus mucronata Willd. (Rhamnaceae) (roots), Lannea schweinfurthii (Engl.) Engl. (Anacardiaceae) (roots), Terminalia sericea Burch. ex DC. (Combretaceae) (roots) and Crinum bulbispermum (Burm.f.) Milne-Redh. & Schweick. (Amaryllidaceae) (roots and bulbs), were shown to possess the ability to inhibit acetylcholinesterase and to contain antioxidant capacity (Adewusi et al., 2011), indicating their potential for use in treatment of neurodegenerative diseases. The aim of this study was to determine whether extracts from these plants are able to reduce neuronal cell death in SH-SY5Y (human neuroblastoma) cells treated with Aβ peptide.
Adewusi et al., AfrJTraditComplementAlternMed.(2013)10(4):6‐11 http://dx.doi.org/10.4314/ajtcam.v10i4.2
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Materials and methods Plant collection and extract preparation The plants investigated include the following: Ziziphus mucronata Willd. (Rhamnaceae) (roots), Lannea schweinfurthii (Engl.) Engl. (Anacardiaceae) (roots), Terminalia sericea Burch. ex DC. (Combretaceae) (roots) and Crinum bulbispermum (Burm.f.) Milne-Redh. & Schweick. (Amaryllidaceae) (roots and bulbs). Z. mucronata (voucher number, NH 1909) and T. sericea (voucher number, NH 1808) were deposited at Soutpanbergensis Herbarium; L. schweinfurthii (voucher number, LT 19) was deposited at Venda, Limpopo, and C. bulbispermum was obtained as a gift from the South African National Biodiversity Institute (SANBI), Tshwane. The plant materials were cut into small pieces, air-dried at room temperature, pulverised and stored at ambient temperature till use. Six grams of the pulverised plant material were extracted with 60 ml of ethanol, while shaking for 24 h. The extracts were filtered and concentrated using a rotary vacuum evaporator and then further dried. All extracts were stored at -20ºC prior to analysis. The dried extracts were re-dissolved in Dimethyl sulfoxide (DMSO) to the desired test concentrations. Cell culture SH-SY5Y cells (ATCC CRL-2266, Rockville, MD, USA) were cultured in Ham’s F-12 supplemented with 2% heatinactivated foetal bovine serum, penicillin (100 U/ml) and streptomycin (100 µg/ml) at 37oC in a humidified incubator at 95% air and 5% CO2. Confluent cells were seeded into 96-well plates at a density of 1 × 105 cells/well. Cell viability MTT assay The 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay as described by Mossmann (1983) was used to measure cell viability. The plated cells were allowed to adhere for 1 h at 37oC after which 20 μl of various concentrations (100, 50, 25, 12.5, 6.25, 3.13, 1.56 and 0.78 μg/ml) of the plant extracts were added. After 72 h of incubation, 20 μl of MTT solution (5 mg/ml) was added to the wells and further incubated for 3 h. 50 μl of solution containing 30% (w/v) N, N dimethylformamide and 20% SDS in water were then added to breach the cells and dissolve the formazan crystals. The plates were maintained overnight in the incubator at 37oC and absorbance was measured at 570-630 nm using a microtiter plate reader (Labsystems Multiscan EX type 355 plate reader). Wells without cells were used as blanks and were subtracted as background from each sample. Cell viability was expressed as a percentage of the control values. Neutral red assay The neutral red uptake assay as described by Borenfreund and Puerner (1984) was also used to assess cell viability. This method is based on the determination of the accumulation of the neutral red dye in the lysosomes of viable, uninjured cells. After treatment and incubation of the cells (as described above for the MTT assay), 150 µl of neutral red dye (100 µg/ml) dissolved in serum free medium (pH 6.4) was added to the culture medium and incubated for 3 h at 37°C. Cells were washed with Phosphate Buffered Saline (PBS), and 150 μl of elution medium (EtOH/AcCOOH, 50%/1%) was added followed by gentle shaking for 60 min, so that complete dissolution could be achieved. Absorbance was recorded at 540-630 nm using a microtiter plate reader. Cell viability was expressed as a percentage of the control values. Treatment with Aβ25‐35 Aβ25-35 was reconstituted in sterile water to a concentration of 1mM. Aliquots were incubated at 37°C for 72 h to form aggregated amyloid. During the experiment, Aβ25-35 was directly added to culture medium to achieve a final concentration of 20 µM. Three concentrations of each of the plant extracts that presented low toxicity (as determined from the tests above) were selected to assess their possible protective effects. The cells were plated as described above and pre-treated with the plant extracts for 2 h before adding Aβ25-35 and then incubated for 72 h. The viability of the cells was determined by the MTT and neutral red assays as described above. Cell viability was expressed as a percentage of the control values. Statistical analysis All determinations were carried out on three occasions in triplicate. The results are reported as mean ± standard deviation (S.D.). Calculation of IC50 values was done using Microsoft Excel 2009 Version for Windows. A p < 0.05 value was considered to be significant.
Results Exposure of the SH-SY5Y cells to Aβ25-35 at 2.5, 5, 10 and 20 µM for 72 h, resulted in a concentration-dependent
Adewusi et al., AfrJTraditComplementAlternMed.(2013)10(4):6‐11 http://dx.doi.org/10.4314/ajtcam.v10i4.2
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decrease in cell survival. Cell viability (expressed as the percentage of control measured in the absence of Aβ) was reduced by 84% (Figure 1) when exposed to 20 µM, and this concentration was therefore used for the determination of Aβ induced neuronal cell damage in the present experiment. The same concentration of Aβ25-35’has been used previously in similar experiments (Lin et al., 2006; Table 1: IC50 values of the methanol extracts of the four plants investigated on untreated SH-SY5Y cells. Plant and part Z. mucronata roots L. schweinfurthii roots T. sericea roots C. bulbispermum roots C. bulbispermum bulbs
Neutral red assay IC50 (µg/ml) >100 >100 >100 10.71 ± 0.26 47.26 ± 0.68
MTT assay IC50 (µg/ml) >100 >100 95.14 ± 2.45 12.53 ± 0.89 48.84 ± 4.62
Liu et al., 2010). The cytotoxic effect of the plant extracts on the viability of the SH-SY5Y cells is presented as IC50 values in Table 1. Values obtained from both the neutral red and MTT assays were comparable. Extracts from the roots of Z. mucronata, L. schweinfurthii and T. sericea were the least toxic with IC50 values exceeding 100 µg/ml, the highest concentration tested, while extracts from the root and bulb of C. bulbispermum showed the highest toxicity as evident from the IC50 values. In order to examine the possible neuroprotective effects of the five plant extracts, three concentrations that presented low toxicity from the cytotoxicity assays, were selected to determine if they could reduce cell death induced by Aβ in SH-SY5Y cells. Pretreatment with Z. mucronata and T. sericea methanol extracts reduced the effect of cell death caused by Aβ25-35 (Fig. 1). An improvement in cell viability, relative to the positive control, was observed with Z. mucronata as 77.7% and 78.7% of viable cells were observed with the neutral red and MTT assays respectively, at 50 µg/ml. Pre-treatment with L. schweinfurthii root extract resulted in an optimum dose for inhibition of Aβ25-35 induced cell death at 25 µg/ml, while still maintaining 80% viability (Fig 1). Extracts of the roots and bulbs of C. bulbispermum were found to reduce the effect of Aβ, but were less effective in comparison with the other plants screened.
Discussion The viability of cells exposed to Aβ25-35 was assessed using the MTT and neutral red uptake assays. These assays are sensitive, quantitative and reliable colorimetric assays for the determination of cell viability (Ban et al., 2006). All four tested plants were observed to reduce the effects of Aβ induced neuronal cell death, indicating that they may contain compounds which may be relevant in the prevention of AD progression. The low toxicity of Z. mucronata as observed in the present study has been reported in literature (McGaw et al. 2007). The authors tested the hexane, methanol and water extracts of the bark and leaf of the plant and obtained IC50 > 100 µg/ml. In addition, the plant also showed a good effect in reducing Aβ induced cell death. Several compounds including spinosin from Z. spinosa; stepharine, asimilobine, quercetin, kaempferol and phloretin derivatives isolated from Z. jujuba have been reported to have neuroprotective activity (Koetter et al., 2009; Chen, 1998; Qian, 1996; Wing, 2001; Pawlowska et al., 2009; Taati et al., 2011). A possible underlying mechanism of the effect observed with Z. mucronata may be associated with the presence of these compounds. The ethanol extract of T. sericea roots showed low cytotoxicity with IC50 value of > 90% in both the neutral red and MTT assays. Similar findings have been reported for T. spinosa as the dichloromethane/methanol (1:1) and 80% ethanol extracts of the stem-bark showed IC50 values of 99.5 µg/ml and 75.8 µg/ml respectively in the brine shrimp assay (Mbwambo et al., 2011). In addition, the present study shows T. sericea to reduce cell death induced by Aβ. Tannic acid, chebulagic acid, chebulinic acid and corilagin, all isolated from T. chebula, have been reported to protect neurons from ischemic damage induced by transient cerebral ischemia, and these or similar compounds may be responsible for the good activity observed with T. sericea (Park et al., 2011). The extracts of the root and bulb of C. bulbispermum were the most toxic with IC50 values
